0	14	Thrombopoietin	Thrombopoietin	NNP
15	23	supports	support	VBZ
24	26	in	in	FW
27	32	vitro	vitro	FW
33	42	erythroid	erythroid	JJ
43	58	differentiation	differentiation	NN
59	62	via	via	IN
63	66	its	its	PRP$
67	75	specific	specific	JJ
76	84	receptor	receptor	NN
85	90	c-Mpl	c-mpl	NN
91	93	in	in	IN
94	95	a	a	DT
96	101	human	human	JJ
102	110	leukemia	leukemia	NN
111	115	cell	cell	NN
116	120	line	line	NN
120	121	.	.	.

123	137	Thrombopoietin	Thrombopoietin	NNP
138	139	(	(	(
139	142	TPO	TPO	NNP
142	143	)	)	)
144	148	acts	act	VBZ
149	151	on	on	IN
152	168	megakaryopoiesis	megakaryopoiesis	NN
169	172	and	and	CC
173	187	erythropoiesis	erythropoiesis	NN
188	190	in	in	FW
191	196	vitro	vitro	FW
197	200	and	and	CC
201	203	in	in	FW
204	208	vivo	vivo	FW
208	209	.	.	.

210	212	We	we	PRP
213	221	isolated	isolate	VBD
222	223	a	a	DT
224	229	novel	novel	JJ
230	237	subline	subline	NN
237	238	,	,	,
239	247	UT-7/GMT	UT-7/GMT	NNP
247	248	,	,	,
249	253	from	from	IN
254	257	the	the	DT
258	263	human	human	JJ
264	272	leukemia	leukemia	NN
273	277	cell	cell	NN
278	282	line	line	NN
283	290	UT-7/GM	UT-7/GM	NNP
291	292	(	(	(
292	294	N.	N.	NNP
295	302	Komatsu	Komatsu	NNP
302	303	,	,	,
304	306	et	et	FW
307	310	al.	al.	FW
310	311	,	,	,
312	317	Blood	Blood	NNP
317	318	,	,	,
319	321	89	89	CD
321	322	:	:	:
323	332	4021-4033	4021-4033	CD
332	333	,	,	,
334	338	1997	1997	CD
338	339	)	)	)
339	340	.	.	.

341	342	A	a	DT
343	348	small	small	JJ
349	359	population	population	NN
360	362	of	of	IN
363	370	UT-7/GM	UT-7/GM	NNP
371	376	cells	cell	NNS
377	387	positively	positively	RB
388	395	stained	stain	VBN
396	399	for	for	IN
400	410	hemoglobin	hemoglobin	NN
411	412	(	(	(
412	414	Hb	Hb	NNP
414	415	)	)	)
416	421	after	after	IN
422	423	a	a	DT
424	429	7-day	7-day	JJ
430	438	exposure	exposure	NN
439	441	to	to	TO
442	445	TPO	TPO	NNP
445	446	.	.	.

447	451	More	more	JJR
452	456	than	than	IN
457	459	50	50	CD
459	460	%	%	NN
461	463	of	of	IN
464	475	TPO-treated	tpo-treated	JJ
476	484	UT-7/GMT	ut-7/gmt	NN
485	490	cells	cell	NNS
491	501	positively	positively	RB
502	509	stained	stain	VBN
510	513	for	for	IN
514	516	Hb	Hb	NNP
516	517	.	.	.

518	523	Using	use	VBG
524	532	UT-7/GMT	ut-7/gmt	NN
533	538	cells	cell	NNS
538	539	,	,	,
540	542	we	we	PRP
543	551	examined	examine	VBD
552	555	how	how	WRB
556	559	TPO	TPO	NNP
560	568	promotes	promote	VBZ
569	586	hemoglobinization	hemoglobinization	NN
586	587	.	.	.

588	591	TPO	TPO	NNP
592	599	induced	induce	VBD
600	608	tyrosine	tyrosine	NN
609	624	phosphorylation	phosphorylation	NN
625	627	of	of	IN
628	631	the	the	DT
632	635	TPO	TPO	NNP
636	644	receptor	receptor	NN
645	648	but	but	CC
649	652	not	not	RB
653	656	the	the	DT
657	671	erythropoietin	erythropoietin	NN
672	673	(	(	(
673	676	EPO	EPO	NNP
676	677	)	)	)
678	686	receptor	receptor	NN
686	687	.	.	.

688	693	There	there	EX
694	697	was	be	VBD
698	700	no	no	DT
701	712	competition	competition	NN
713	720	between	between	IN
721	724	TPO	TPO	NNP
725	728	and	and	CC
729	732	EPO	EPO	NNP
733	736	for	for	IN
737	744	binding	binding	NN
745	747	to	to	TO
748	751	EPO	EPO	NNP
752	760	receptor	receptor	NN
760	761	.	.	.

762	767	These	these	DT
768	776	findings	finding	NNS
777	784	suggest	suggest	VBP
785	789	that	that	IN
790	793	TPO	TPO	NNP
794	797	has	have	VBZ
798	799	a	a	DT
800	806	direct	direct	JJ
807	813	effect	effect	NN
814	816	on	on	IN
817	834	hemoglobinization	hemoglobinization	NN
835	838	via	via	IN
839	840	a	a	DT
841	849	specific	specific	JJ
850	858	receptor	receptor	NN
859	861	on	on	IN
862	870	UT-7/GMT	UT-7/GMT	NNP
871	876	cells	cell	NNS
876	877	.	.	.

878	889	Isoelectric	isoelectric	JJ
890	898	focusing	focusing	NN
899	911	demonstrated	demonstrate	VBD
912	916	that	that	IN
917	920	TPO	TPO	NNP
921	928	induced	induce	VBD
929	934	fetal	fetal	JJ
935	938	and	and	CC
939	944	adult	adult	JJ
945	947	Hb	Hb	NNP
948	957	synthesis	synthesis	NN
957	958	,	,	,
959	966	whereas	whereas	IN
967	970	EPO	EPO	NNP
971	978	induced	induce	VBD
979	988	embryonic	embryonic	JJ
988	989	,	,	,
990	995	fetal	fetal	JJ
995	996	,	,	,
997	1000	and	and	CC
1001	1006	adult	adult	JJ
1007	1009	Hb	Hb	NNP
1010	1019	synthesis	synthesis	NN
1019	1020	.	.	.

1021	1025	Thus	thus	RB
1025	1026	,	,	,
1027	1030	our	our	PRP$
1031	1035	data	datum	NNS
1036	1043	suggest	suggest	VBP
1044	1048	that	that	IN
1049	1052	TPO	TPO	NNP
1053	1056	has	have	VBZ
1057	1058	a	a	DT
1059	1067	distinct	distinct	JJ
1068	1074	action	action	NN
1075	1077	on	on	IN
1078	1092	erythropoiesis	erythropoiesis	NN
1092	1093	.	.	.