0 7 Nuclear nuclear JJ 8 21 factor-kappaB factor-kappaB NNP 22 31 induction induction NN 32 34 in in IN 35 42 CD45RO+ cd45ro+ JJ 43 46 and and CC 47 54 CD45RA+ cd45ra+ JJ 55 56 T t NN 57 61 cell cell NN 62 69 subsets subset NNS 70 76 during during IN 77 82 aging aging NN 82 83 . . . 85 87 An an DT 88 96 increase increase NN 97 99 in in IN 100 103 the the DT 104 109 ratio ratio NN 110 112 of of IN 113 119 memory memory NN 120 122 to to TO 123 128 naive naive JJ 129 130 T t NN 131 136 cells cell NNS 137 140 has have VBZ 141 145 been be VBN 146 156 postulated postulate VBN 157 159 to to TO 160 168 underlie underlie VB 169 175 immune immune JJ 176 194 hyporesponsiveness hyporesponsiveness NN 195 207 accompanying accompany VBG 208 213 aging aging NN 213 214 . . . 215 218 Our our PRP$ 219 227 analyses analysis NNS 228 230 of of IN 231 234 the the DT 235 244 induction induction NN 245 247 of of IN 248 255 nuclear nuclear JJ 256 269 factor-kappaB factor-kappaB NNP 270 271 ( ( ( 271 279 NFkappaB NFkappaB NNP 279 280 ) ) ) 281 283 in in IN 284 293 activated activate VBN 294 300 memory memory NN 301 302 ( ( ( 302 309 CD45RO+ CD45RO+ NNP 309 310 ) ) ) 311 314 and and CC 315 320 naive naive JJ 321 322 ( ( ( 322 329 CD45RA+ cd45ra+ JJ 329 330 ) ) ) 331 332 T t NN 333 337 cell cell NN 338 345 subsets subset NNS 346 350 from from IN 351 356 young young JJ 357 360 and and CC 361 368 elderly elderly JJ 369 375 donors donor NNS 376 379 has have VBZ 380 392 demonstrated demonstrate VBN 393 397 that that IN 397 398 , , , 399 409 regardless regardless RB 410 412 of of IN 413 418 donor donor NN 419 422 age age NN 422 423 , , , 424 430 memory memory NN 431 432 T t NN 433 438 cells cell NNS 439 442 are be VBP 443 446 not not RB 447 460 significantly significantly RB 461 468 altered alter VBN 469 471 in in IN 472 477 their their PRP$ 478 492 responsiveness responsiveness NN 493 495 to to TO 496 514 TNF-alpha-mediated tnf-alpha-mediated JJ 515 524 induction induction NN 525 527 of of IN 528 536 NFkappaB NFkappaB NNP 536 537 . . . 538 546 Although although IN 547 556 treatment treatment NN 557 561 with with IN 562 571 TNF-alpha TNF-alpha NNP 572 579 induced induce VBD 580 587 nuclear nuclear JJ 588 600 localization localization NN 601 603 of of IN 604 612 NFkappaB NFkappaB NNP 613 615 in in IN 616 620 both both CC 621 627 memory memory NN 628 631 and and CC 632 637 naive naive JJ 638 639 T t NN 640 644 cell cell NN 645 652 subsets subset NNS 652 653 , , , 654 666 irrespective irrespective JJ 667 669 of of IN 670 673 the the DT 674 677 age age NN 678 680 of of IN 681 684 the the DT 685 690 donor donor NN 690 691 , , , 692 695 the the DT 696 702 levels level NNS 703 705 of of IN 706 713 induced induce VBN 714 722 NFkappaB NFkappaB NNP 723 727 were be VBD 728 741 significantly significantly RB 742 747 lower low JJR 748 750 in in IN 751 755 both both DT 756 763 subsets subset NNS 764 766 of of IN 767 768 T t NN 769 774 cells cell NNS 775 783 obtained obtain VBN 784 788 from from IN 789 792 the the DT 793 800 elderly elderly JJ 800 801 , , , 802 806 when when WRB 807 815 compared compare VBN 816 818 to to TO 819 824 those those DT 825 827 in in IN 828 833 young young JJ 833 834 . . . 835 846 Examination Examination NNP 847 849 of of IN 850 857 IkappaB IkappaB NNP 858 863 alpha alpha NN 864 874 regulation regulation NN 875 883 revealed reveal VBD 884 888 that that IN 889 907 TNF-alpha-mediated tnf-alpha-mediated JJ 908 919 degradation degradation NN 920 922 of of IN 923 930 IkappaB IkappaB NNP 931 936 alpha alpha NN 937 939 in in IN 940 944 both both CC 945 951 memory memory NN 952 955 and and CC 956 961 naive naive JJ 962 963 T t NN 964 969 cells cell NNS 970 974 from from IN 975 978 the the DT 979 986 elderly elderly JJ 987 990 was be VBD 991 999 severely severely RB 1000 1008 impaired impaired JJ 1008 1009 , , , 1010 1014 thus thus RB 1015 1027 contributing contribute VBG 1028 1030 to to TO 1031 1034 the the DT 1035 1042 lowered lowered JJ 1043 1052 induction induction NN 1053 1055 of of IN 1056 1059 the the DT 1060 1068 observed observe VBN 1069 1077 NFkappaB nfkappab NN 1077 1078 . . . 1079 1081 In in IN 1082 1090 addition addition NN 1090 1091 , , , 1092 1096 this this DT 1097 1108 age-related age-related JJ 1109 1117 decrease decrease NN 1118 1120 in in IN 1121 1130 induction induction NN 1131 1133 of of IN 1134 1141 nuclear nuclear JJ 1142 1150 NFkappaB nfkappab NN 1151 1161 correlated correlate VBD 1162 1166 with with IN 1167 1175 decrease decrease NN 1176 1178 in in IN 1179 1192 intracellular intracellular JJ 1193 1197 IL-2 il-2 NN 1198 1206 receptor receptor NN 1207 1217 expression expression NN 1218 1221 and and CC 1222 1238 anti-CD3-induced anti-cd3-induced JJ 1239 1252 proliferation proliferation NN 1253 1255 of of IN 1256 1260 both both CC 1261 1267 memory memory NN 1268 1271 and and CC 1272 1277 naive naive JJ 1278 1279 T t NN 1280 1285 cells cell NNS 1286 1293 subsets subset NNS 1293 1294 . . . 1295 1300 Taken take VBN 1301 1309 together together RB 1309 1310 , , , 1311 1314 our our PRP$ 1315 1322 results result NNS 1323 1330 suggest suggest VBP 1331 1335 that that IN 1336 1339 the the DT 1340 1351 age-related age-related JJ 1352 1370 hyporesponsiveness hyporesponsiveness NN 1371 1377 cannot cannot MD 1378 1380 be be VB 1381 1391 attributed attribute VBN 1392 1394 to to TO 1395 1396 a a DT 1397 1404 skewing skewing NN 1405 1407 of of IN 1408 1411 the the DT 1412 1413 T t NN 1414 1418 cell cell NN 1419 1429 population population NN 1430 1437 towards towards IN 1438 1439 a a DT 1440 1446 memory memory NN 1447 1456 phenotype phenotype NN 1457 1459 in in IN 1460 1463 the the DT 1464 1471 elderly elderly JJ 1471 1472 . . .