0 13 Transcription transcription NN 14 16 of of IN 17 18 a a DT 19 26 minimal minimal JJ 27 35 promoter promoter NN 36 40 from from IN 41 44 the the DT 45 51 NF-IL6 NF-IL6 NNP 52 56 gene gene NN 57 59 is be VBZ 60 69 regulated regulate VBN 70 72 by by IN 73 81 CREB/ATF CREB/ATF NNP 82 85 and and CC 86 89 SP1 sp1 NN 90 98 proteins protein NNS 99 101 in in IN 102 106 U937 u937 NN 107 119 promonocytic promonocytic JJ 120 125 cells cell NNS 125 126 . . . 128 134 NF-IL6 NF-IL6 NNP 135 137 is be VBZ 138 140 an an DT 141 150 important important JJ 151 166 transcriptional transcriptional JJ 167 176 regulator regulator NN 177 179 of of IN 180 185 genes gene NNS 186 193 induced induce VBN 194 196 in in IN 197 206 activated activate VBN 207 228 monocytes/macrophages monocytes/macrophage NNS 228 229 , , , 230 233 and and CC 234 240 NF-IL6 NF-IL6 NNP 241 243 is be VBZ 244 247 the the DT 248 252 only only RB 253 275 CCAAT/enhancer-binding ccaat/enhancer-binding JJ 276 283 protein protein NN 284 285 ( ( ( 285 290 C/EBP C/EBP NNP 290 291 ) ) ) 292 298 family family NN 299 305 member member NN 306 311 whose whose WP$ 312 324 steady-state steady-state JJ 325 329 mRNA mrna NN 330 336 levels level NNS 337 345 increase increase VBP 346 350 upon upon IN 351 361 activation activation NN 362 364 of of IN 365 374 monocytes monocyte NNS 375 376 ( ( ( 376 377 1 1 CD 377 378 ) ) ) 378 379 . . . 380 382 We we PRP 383 387 show show VBP 388 392 that that IN 393 402 increased increase VBN 403 416 transcription transcription NN 417 419 of of IN 420 423 the the DT 424 430 NF-IL6 NF-IL6 NNP 431 435 gene gene NN 436 438 is be VBZ 439 450 responsible responsible JJ 450 451 , , , 452 454 at at IN 455 460 least least JJS 461 463 in in IN 464 468 part part NN 468 469 , , , 470 473 for for IN 474 483 induction induction NN 484 486 of of IN 487 493 NF-IL6 NF-IL6 NNP 494 498 mRNA mRNA NNP 499 508 following follow VBG 509 519 activation activation NN 520 522 of of IN 523 527 U937 u937 NN 528 540 promonocytic promonocytic JJ 541 546 cells cell NNS 546 547 . . . 548 550 We we PRP 551 555 have have VBP 556 566 identified identify VBN 567 568 a a DT 569 575 104-bp 104-bp JJ 576 583 minimal minimal JJ 584 592 promoter promoter NN 593 599 region region NN 600 602 of of IN 603 606 the the DT 607 613 NF-IL6 NF-IL6 NNP 614 618 gene gene NN 619 623 that that WDT 624 626 is be VBZ 627 637 sufficient sufficient JJ 638 641 for for IN 642 647 basal basal JJ 648 651 and and CC 652 672 activation-dependent activation-dependent JJ 673 682 induction induction NN 683 685 of of IN 686 699 transcription transcription NN 700 702 in in IN 703 707 U937 u937 NN 708 713 cells cell NNS 713 714 . . . 715 719 This this DT 720 726 region region NN 727 735 contains contain VBZ 736 743 binding bind VBG 744 749 sites site NNS 750 753 for for IN 754 757 the the DT 758 762 cAMP camp NN 763 771 response response NN 772 787 element-binding element-binding JJ 788 806 protein/activation protein/activation NN 807 820 transcription transcription NN 821 827 factor factor NN 828 829 ( ( ( 829 837 CREB/ATF CREB/ATF NNP 837 838 ) ) ) 839 842 and and CC 843 846 Sp1 sp1 NN 847 855 families family NNS 856 858 of of IN 859 872 transcription transcription NN 873 880 factors factor NNS 880 881 . . . 882 886 Each each DT 887 891 site site NN 892 894 is be VBZ 895 907 functionally functionally RB 908 917 important important JJ 918 921 and and CC 922 933 contributes contribute VBZ 934 947 independently independently RB 948 950 to to TO 951 964 transcription transcription NN 965 967 of of IN 968 971 the the DT 972 978 NF-IL6 NF-IL6 NNP 979 983 gene gene NN 984 986 in in IN 987 991 U937 u937 NN 992 997 cells cell NNS 997 998 . . .