0 9 Activated activate VBN 10 19 platelets platelet NNS 20 26 induce induce VBP 27 35 monocyte monocyte NN 36 47 chemotactic chemotactic JJ 48 57 protein-1 protein-1 NN 58 67 secretion secretion NN 68 71 and and CC 72 79 surface surface NN 80 90 expression expression NN 91 93 of of IN 94 107 intercellular intercellular JJ 108 116 adhesion adhesion NN 117 127 molecule-1 molecule-1 NN 128 130 on on IN 131 142 endothelial endothelial JJ 143 148 cells cell NNS 149 150 [ [ ( 150 153 see see VB 154 162 comments comment NNS 162 163 ] ] ) 165 175 BACKGROUND BACKGROUND NNP 175 176 : : : 177 197 Platelet/endothelium platelet/endothelium NN 198 209 interaction interaction NN 210 215 plays play VBZ 216 218 an an DT 219 228 important important JJ 229 233 role role NN 234 236 in in IN 237 240 the the DT 241 256 pathophysiology pathophysiology NN 257 259 of of IN 260 272 inflammation inflammation NN 273 276 and and CC 277 292 atherosclerosis atherosclerosis NN 292 293 . . . 294 297 The the DT 298 302 role role NN 303 305 of of IN 306 315 platelets platelet NNS 316 319 for for IN 320 328 monocyte monocyte NN 329 340 chemotactic chemotactic JJ 341 350 protein-1 protein-1 NN 351 352 ( ( ( 352 357 MCP-1 mcp-1 NN 357 358 ) ) ) 359 368 secretion secretion NN 369 372 and and CC 373 380 surface surface NN 381 391 expression expression NN 392 394 of of IN 395 408 intercellular intercellular JJ 409 417 adhesion adhesion NN 418 428 molecule-1 molecule-1 NN 429 430 ( ( ( 430 436 ICAM-1 icam-1 NN 436 437 ) ) ) 438 440 on on IN 441 452 endothelial endothelial JJ 453 458 cells cell NNS 459 462 has have VBZ 463 467 been be VBN 468 476 assessed assess VBN 476 477 . . . 478 485 METHODS methods NNS 486 489 AND and CC 490 497 RESULTS results NNS 497 498 : : : 499 509 Monolayers monolayer NNS 510 512 of of IN 513 518 human human JJ 519 528 umbilical umbilical JJ 529 533 vein vein NN 534 545 endothelial endothelial JJ 546 551 cells cell NNS 552 556 were be VBD 557 566 incubated incubate VBN 567 571 with with IN 572 585 nonstimulated nonstimulated JJ 586 588 or or CC 589 602 ADP-activated adp-activated JJ 603 612 platelets platelet NNS 613 616 for for IN 617 618 6 6 CD 619 624 hours hour NNS 624 625 , , , 626 629 and and CC 630 639 secretion secretion NN 640 642 of of IN 643 648 MCP-1 mcp-1 NN 649 652 and and CC 653 660 surface surface NN 661 671 expression expression NN 672 674 of of IN 675 681 ICAM-1 ICAM-1 NNP 682 686 were be VBD 687 697 determined determine VBN 698 700 by by IN 701 706 ELISA ELISA NNP 707 710 and and CC 711 715 flow flow NN 716 725 cytometry cytometry NN 725 726 , , , 727 739 respectively respectively RB 739 740 . . . 741 743 In in IN 744 747 the the DT 748 756 presence presence NN 757 759 of of IN 760 773 ADP-activated adp-activated JJ 774 783 platelets platelet NNS 783 784 , , , 785 789 both both CC 790 795 MCP-1 mcp-1 NN 796 805 secretion secretion NN 806 809 and and CC 810 816 ICAM-1 icam-1 NN 817 824 surface surface NN 825 835 expression expression NN 836 840 were be VBD 841 854 significantly significantly RB 855 864 increased increase VBN 865 873 compared compare VBN 874 878 with with IN 879 892 nonstimulated nonstimulated JJ 893 902 platelets platelet NNS 903 904 ( ( ( 904 910 P<0.02 p<0.02 NN 910 911 ) ) ) 911 912 . . . 913 923 Activation Activation NNP 924 926 of of IN 927 930 the the DT 931 944 transcription transcription NN 945 951 factor factor NN 952 959 nuclear nuclear JJ 960 973 factor-kappaB factor-kappaB NNP 974 975 ( ( ( 975 984 NF-kappaB NF-kappaB NNP 984 985 ) ) ) 986 996 determined determine VBN 997 999 by by IN 1000 1015 electrophoretic electrophoretic JJ 1016 1024 mobility mobility NN 1025 1030 shift shift NN 1031 1036 assay assay NN 1037 1040 and and CC 1041 1057 kappaB-dependent kappab-dependent JJ 1058 1073 transcriptional transcriptional JJ 1074 1082 activity activity NN 1083 1086 was be VBD 1087 1095 enhanced enhance VBN 1096 1098 in in IN 1099 1102 the the DT 1103 1111 presence presence NN 1112 1114 of of IN 1115 1124 activated activate VBN 1125 1134 platelets platelet NNS 1134 1135 . . . 1136 1138 In in IN 1139 1147 addition addition NN 1147 1148 , , , 1149 1162 ADP-activated adp-activated JJ 1163 1172 platelets platelet NNS 1173 1180 induced induce VBD 1181 1186 MCP-1 mcp-1 NN 1187 1190 and and CC 1191 1197 ICAM-1 icam-1 NN 1198 1216 promoter-dependent promoter-dependent JJ 1217 1230 transcription transcription NN 1230 1231 . . . 1232 1241 Liposomal liposomal JJ 1242 1254 transfection transfection NN 1255 1257 of of IN 1258 1259 a a DT 1260 1275 double-stranded double-stranded JJ 1276 1282 kappaB kappab NN 1283 1299 phosphorothioate phosphorothioate NN 1300 1315 oligonucleotide oligonucleotide NN 1315 1316 , , , 1317 1320 but but CC 1321 1324 not not RB 1325 1327 of of IN 1328 1331 the the DT 1332 1339 mutated mutate VBN 1340 1344 form form NN 1344 1345 , , , 1346 1355 inhibited inhibit VBD 1356 1361 MCP-1 mcp-1 NN 1362 1371 secretion secretion NN 1372 1375 and and CC 1376 1383 surface surface NN 1384 1394 expression expression NN 1395 1397 of of IN 1398 1404 ICAM-1 icam-1 NN 1405 1407 on on IN 1408 1417 activated activate VBN 1418 1429 endothelium endothelium NN 1430 1431 ( ( ( 1431 1437 P<0.05 p<0.05 NN 1437 1438 ) ) ) 1438 1439 . . . 1440 1451 CONCLUSIONS conclusions NNS 1451 1452 : : : 1453 1456 The the DT 1457 1464 present present JJ 1465 1470 study study NN 1471 1480 indicates indicate VBZ 1481 1485 that that IN 1486 1495 activated activate VBN 1496 1505 platelets platelet NNS 1506 1514 modulate modulate VBP 1515 1526 chemotactic chemotactic JJ 1527 1528 ( ( ( 1528 1533 MCP-1 mcp-1 NN 1533 1534 ) ) ) 1535 1538 and and CC 1539 1547 adhesive adhesive JJ 1548 1549 ( ( ( 1549 1555 ICAM-1 icam-1 NN 1555 1556 ) ) ) 1557 1567 properties property NNS 1568 1570 of of IN 1571 1582 endothelial endothelial JJ 1583 1588 cells cell NNS 1589 1592 via via IN 1593 1595 an an DT 1596 1615 NF-kappaB-dependent nf-kappab-dependent JJ 1616 1625 mechanism mechanism NN 1625 1626 . . . 1627 1643 Platelet-induced platelet-induced JJ 1644 1654 activation activation NN 1655 1657 of of IN 1658 1661 the the DT 1662 1671 NF-kappaB NF-kappaB NNP 1672 1678 system system NN 1679 1684 might might MD 1685 1695 contribute contribute VB 1696 1698 to to TO 1699 1704 early early JJ 1705 1717 inflammatory inflammatory JJ 1718 1724 events event NNS 1725 1727 in in IN 1728 1741 atherogenesis atherogenesis NN 1741 1742 . . .