0 10 Activation Activation NNP 11 13 of of IN 14 22 distinct distinct JJ 23 36 transcription transcription NN 37 44 factors factor NNS 45 47 in in IN 48 59 neutrophils neutrophil NNS 60 62 by by IN 63 72 bacterial bacterial JJ 73 76 LPS lps NN 76 77 , , , 78 94 interferon-gamma interferon-gamma NN 94 95 , , , 96 99 and and CC 100 106 GM-CSF GM-CSF NNP 107 110 and and CC 111 114 the the DT 115 124 necessity necessity NN 125 127 to to TO 128 136 overcome overcome VB 137 140 the the DT 141 147 action action NN 148 150 of of IN 151 161 endogenous endogenous JJ 162 171 proteases protease NNS 171 172 . . . 174 179 Human human JJ 180 191 neutrophils neutrophil NNS 192 195 can can MD 196 198 be be VB 199 206 induced induce VBN 207 209 to to TO 210 218 actively actively RB 219 229 transcribe transcribe VB 230 231 a a DT 232 238 number number NN 239 241 of of IN 242 256 early-response early-response JJ 257 262 genes gene NNS 262 263 , , , 264 266 in in IN 267 277 particular particular JJ 278 283 those those DT 284 292 encoding encode VBG 293 302 cytokines cytokine NNS 302 303 , , , 304 314 chemokines chemokine NNS 314 315 , , , 316 319 and and CC 320 323 the the DT 324 337 high-affinity high-affinity JJ 338 345 surface surface NN 346 354 receptor receptor NN 355 358 for for IN 359 362 IgG IgG NNP 362 363 , , , 364 373 FcgammaRI FcgammaRI NNP 373 374 . . . 375 383 Although although IN 384 390 little little JJ 391 393 is be VBZ 394 399 known know VBN 400 402 to to TO 403 407 date date NN 408 413 about about IN 414 417 the the DT 418 428 regulation regulation NN 429 431 of of IN 432 436 gene gene NN 437 450 transcription transcription NN 451 453 in in IN 454 465 neutrophils neutrophil NNS 465 466 , , , 467 474 several several JJ 475 486 indications indication NNS 487 492 point point VBP 493 495 to to TO 496 497 a a DT 498 502 role role NN 503 506 for for IN 507 515 distinct distinct JJ 516 529 transcription transcription NN 530 537 factors factor NNS 537 538 , , , 539 543 such such JJ 544 546 as as IN 547 554 members member NNS 555 557 of of IN 558 561 the the DT 562 571 NF-kappaB NF-kappaB NNP 572 575 and and CC 576 580 STAT STAT NNP 581 589 families family NNS 589 590 . . . 591 593 In in IN 594 598 this this DT 599 604 study study NN 604 605 , , , 606 608 we we PRP 609 621 investigated investigate VBD 622 629 whether whether IN 630 635 these these DT 636 649 transcription transcription NN 650 657 factors factor NNS 658 664 become become VBP 665 674 activated activate VBN 675 680 under under IN 681 692 stimulatory stimulatory JJ 693 703 conditions condition NNS 704 709 which which WDT 710 713 are be VBP 714 719 known know VBN 720 722 to to TO 723 729 induce induce VB 730 734 gene gene NN 735 748 transcription transcription NN 749 751 in in IN 752 763 neutrophils neutrophil NNS 763 764 . . . 765 777 Unexpectedly unexpectedly RB 777 778 , , , 779 781 we we PRP 782 787 found find VBD 788 792 that that IN 793 805 conventional conventional JJ 806 816 procedures procedure NNS 817 825 employed employ VBN 826 828 to to TO 829 836 prepare prepare VB 837 845 cellular cellular JJ 846 854 extracts extract NNS 855 860 cause cause VBP 861 864 the the DT 865 872 release release NN 873 875 of of IN 876 887 proteolytic proteolytic JJ 888 898 activities activity NNS 899 903 that that WDT 904 907 are be VBP 908 916 normally normally RB 917 923 stored store VBN 924 926 in in IN 927 940 intracellular intracellular JJ 941 949 granules granule NNS 949 950 , , , 951 960 resulting result VBG 961 963 in in IN 964 967 the the DT 968 979 degradation degradation NN 980 982 of of IN 983 990 various various JJ 991 1004 NF-kappaB/Rel NF-kappaB/Rel NNP 1005 1008 and and CC 1009 1013 STAT STAT NNP 1014 1022 proteins protein NNS 1022 1023 . . . 1024 1026 To to TO 1027 1037 circumvent circumvent VB 1038 1042 this this DT 1043 1050 problem problem NN 1050 1051 , , , 1052 1054 we we PRP 1055 1064 developed develop VBD 1065 1067 an an DT 1068 1079 alternative alternative JJ 1080 1089 procedure procedure NN 1090 1095 which which WDT 1096 1103 allowed allow VBD 1104 1106 us us PRP 1107 1109 to to TO 1110 1114 show show VB 1115 1119 that that IN 1120 1122 in in IN 1123 1134 neutrophils neutrophil NNS 1134 1135 , , , 1136 1139 LPS LPS NNP 1140 1143 and and CC 1144 1152 TNFalpha TNFalpha NNP 1153 1159 induce induce VBP 1160 1161 a a DT 1162 1171 NF-kappaB NF-kappaB NNP 1172 1183 DNA-binding dna-binding JJ 1184 1192 activity activity NN 1193 1198 which which WDT 1199 1210 essentially essentially RB 1211 1219 consists consist VBZ 1220 1222 of of IN 1223 1231 p50/RelA p50/rela NN 1232 1238 dimers dimer NNS 1238 1239 , , , 1240 1243 and and CC 1244 1248 that that IN 1249 1257 IFNgamma IFNgamma NNP 1258 1266 promotes promote VBZ 1267 1270 the the DT 1271 1278 binding binding NN 1279 1281 of of IN 1282 1287 STAT1 stat1 NN 1288 1298 homodimers homodimer NNS 1299 1301 to to TO 1302 1305 the the DT 1306 1314 IFNgamma IFNgamma NNP 1315 1323 response response NN 1324 1330 region region NN 1331 1333 of of IN 1334 1337 the the DT 1338 1347 FcgammaRI FcgammaRI NNP 1348 1356 promoter promoter NN 1356 1357 . . . 1358 1366 Moreover moreover RB 1366 1367 , , , 1368 1370 we we PRP 1371 1377 report report VBP 1378 1382 that that IN 1383 1393 neutrophil neutrophil NN 1394 1405 stimulation stimulation NN 1406 1410 with with IN 1411 1417 GM-CSF GM-CSF NNP 1418 1425 results result VBZ 1426 1428 in in IN 1429 1432 the the DT 1433 1442 formation formation NN 1443 1445 of of IN 1446 1447 a a DT 1448 1464 STAT5-containing stat5-containing JJ 1465 1476 DNA-binding dna-binding JJ 1477 1485 activity activity NN 1485 1486 . . . 1487 1499 Collectively collectively RB 1499 1500 , , , 1501 1504 the the DT 1505 1512 current current JJ 1513 1521 findings finding NNS 1522 1526 open open VBP 1527 1530 new new JJ 1531 1543 perspectives perspective NNS 1544 1549 about about IN 1550 1560 mechanisms mechanism NNS 1561 1565 that that WDT 1566 1569 are be VBP 1570 1576 likely likely JJ 1577 1579 to to TO 1580 1588 regulate regulate VB 1589 1593 gene gene NN 1594 1607 transcription transcription NN 1608 1610 in in IN 1611 1622 neutrophils neutrophil NNS 1622 1623 . . . 1624 1626 In in IN 1627 1635 addition addition NN 1635 1636 , , , 1637 1640 the the DT 1641 1650 procedure procedure NN 1651 1660 described describe VBN 1661 1667 herein herein RB 1668 1673 could could MD 1674 1679 prove prove VB 1680 1686 useful useful JJ 1687 1689 in in IN 1690 1695 other other JJ 1696 1700 cell cell NN 1701 1706 types type NNS 1707 1711 that that WDT 1712 1719 express express VBP 1720 1724 high high JJ 1725 1731 levels level NNS 1732 1734 of of IN 1735 1745 endogenous endogenous JJ 1746 1755 proteases protease NNS 1755 1756 . . .