0 12 Differential differential JJ 13 16 RNA RNA NNP 17 24 display display NN 25 35 identifies identify VBZ 36 41 novel novel JJ 42 47 genes gene NNS 48 58 associated associate VBN 59 63 with with IN 64 73 decreased decrease VBN 74 81 vitamin vitamin NN 82 83 D D NNP 84 92 receptor receptor NN 93 103 expression expression NN 103 104 . . . 106 108 To to TO 109 121 characterize characterize VB 122 129 further further RBR 130 133 the the DT 134 142 function function NN 143 145 of of IN 146 149 the the DT 150 163 intracellular intracellular JJ 164 171 vitamin vitamin NN 172 173 D D NNP 174 182 receptor receptor NN 183 184 ( ( ( 184 187 VDR VDR NNP 187 188 ) ) ) 188 189 , , , 190 192 we we PRP 193 197 have have VBP 198 207 developed develop VBN 208 214 stable stable JJ 215 227 transfectant transfectant JJ 228 236 variants variant NNS 237 239 of of IN 240 241 a a DT 242 249 vitamin vitamin NN 250 262 D-responsive d-responsive JJ 263 267 cell cell NN 268 272 line line NN 273 274 ( ( ( 274 278 U937 u937 NN 278 279 ) ) ) 280 285 which which WDT 286 293 express express VBP 294 300 either either CC 301 310 decreased decrease VBN 311 313 or or CC 314 323 increased increase VBN 324 331 numbers number NNS 332 334 of of IN 335 338 VDR VDR NNP 338 339 . . . 340 342 In in IN 343 347 this this DT 348 353 study study NN 354 356 we we PRP 357 361 have have VBP 362 370 analyzed analyze VBN 371 378 changes change NNS 379 381 in in IN 382 386 gene gene NN 387 397 expression expression NN 398 408 associated associate VBN 409 413 with with IN 414 418 this this DT 419 427 variable variable JJ 428 431 VDR vdr NN 432 442 expression expression NN 442 443 . . . 444 451 Initial initial JJ 452 463 experiments experiment NNS 464 473 indicated indicate VBD 474 478 that that IN 479 480 a a DT 481 483 50 50 CD 483 484 % % NN 485 493 decrease decrease NN 494 496 in in IN 497 500 VDR VDR NNP 501 507 levels level NNS 508 511 was be VBD 512 522 associated associate VBN 523 527 with with IN 528 529 a a DT 530 536 2-fold 2-fold JJ 537 545 increase increase NN 546 548 in in IN 549 553 cell cell NN 554 567 proliferation proliferation NN 568 571 and and CC 572 573 a a DT 574 581 similar similar JJ 582 586 rise rise NN 587 589 in in IN 590 595 c-myc c-myc NN 596 600 mRNA mrna NN 601 611 expression expression NN 611 612 . . . 613 620 Further further JJ 621 628 studies study NNS 629 633 were be VBD 634 641 carried carry VBN 642 645 out out RP 646 651 using use VBG 652 664 differential differential JJ 665 668 RNA RNA NNP 669 676 display display NN 677 678 ( ( ( 678 680 DD DD NNP 680 681 ) ) ) 681 682 . . . 683 691 Sequence sequence NN 692 700 analysis analysis NN 701 703 of of IN 704 706 DD DD NNP 707 715 products product NNS 716 724 revealed reveal VBD 725 728 two two CD 729 734 cDNAs cdna NNS 735 739 with with IN 740 748 identity identity NN 749 751 to to TO 752 757 known know VBN 758 762 gene gene NN 763 771 products product NNS 771 772 : : : 773 776 the the DT 777 786 catalytic catalytic JJ 787 795 sub-unit sub-unit NN 796 798 of of IN 799 810 DNA-protein dna-protein JJ 811 817 kinase kinase NNP 818 819 ( ( ( 819 830 DNA-PK(CS)) DNA-PK(CS)) NNP 830 831 , , , 832 835 and and CC 836 839 the the DT 840 851 peroxisomal peroxisomal JJ 852 858 enzyme enzyme NN 859 880 17beta-hydroxysteroid 17beta-hydroxysteroid NN 881 894 dehydrogenase dehydrogenase NN 895 899 type type NN 900 902 IV iv CD 903 904 ( ( ( 904 914 17beta-HSD 17beta-hsd NN 915 917 IV iv CD 917 918 ) ) ) 918 919 . . . 920 928 Northern Northern NNP 929 937 analysis analysis NN 938 947 confirmed confirm VBD 948 952 that that IN 953 963 expression expression NN 964 966 of of IN 967 971 both both DT 972 977 mRNAs mrna NNS 978 981 was be VBD 982 989 reduced reduce VBN 990 992 in in IN 993 998 cells cell NNS 999 1003 with with IN 1004 1013 decreased decrease VBN 1014 1021 numbers number NNS 1022 1024 of of IN 1025 1028 VDR VDR NNP 1028 1029 . . . 1030 1045 Down-regulation down-regulation NN 1046 1048 of of IN 1049 1059 17beta-HSD 17beta-hsd NN 1060 1062 IV iv CD 1063 1067 mRNA mRNA NNP 1068 1078 expression expression NN 1079 1082 was be VBD 1083 1093 associated associate VBN 1094 1098 with with IN 1099 1107 enhanced enhance VBN 1108 1117 estradiol estradiol NN 1118 1130 inactivation inactivation NN 1131 1133 by by IN 1134 1138 U937 u937 NN 1139 1144 cells cell NNS 1144 1145 , , , 1146 1156 suggesting suggest VBG 1157 1158 a a DT 1159 1163 link link NN 1164 1171 between between IN 1172 1182 estrogenic estrogenic JJ 1183 1191 pathways pathway NNS 1192 1195 and and CC 1196 1200 cell cell NN 1201 1214 proliferation proliferation NN 1214 1215 . . . 1216 1223 Further further JJ 1224 1232 Northern northern NN 1233 1241 analyses analysis NNS 1242 1251 indicated indicate VBD 1252 1256 that that IN 1257 1262 there there EX 1263 1266 was be VBD 1267 1269 no no DT 1270 1281 significant significant JJ 1282 1288 change change NN 1289 1291 in in IN 1292 1302 17beta-HSD 17beta-hsd NN 1303 1305 IV iv CD 1306 1308 or or CC 1309 1319 DNA-PK(CS) DNA-PK(CS) NNP 1320 1324 mRNA mrna NN 1325 1331 levels level NNS 1332 1341 following follow VBG 1342 1351 treatment treatment NN 1352 1356 with with IN 1357 1368 1,25(OH)2D3 1,25(OH)2D3 NNP 1368 1369 , , , 1370 1378 although although IN 1379 1389 expression expression NN 1390 1392 of of IN 1393 1397 both both DT 1398 1403 genes gene NNS 1404 1410 varied vary VBD 1411 1415 with with IN 1416 1423 changes change NNS 1424 1426 in in IN 1427 1431 cell cell NN 1432 1445 proliferation proliferation NN 1445 1446 . . . 1447 1452 These these DT 1453 1457 data datum NNS 1458 1465 suggest suggest VBP 1466 1470 that that IN 1470 1471 , , , 1472 1474 in in IN 1475 1483 addition addition NN 1484 1486 to to TO 1487 1490 its its PRP$ 1491 1502 established established JJ 1503 1507 role role NN 1508 1510 as as IN 1511 1512 a a DT 1513 1530 hormone-dependent hormone-dependent JJ 1531 1546 trans-activator trans-activator NN 1546 1547 , , , 1548 1551 VDR VDR NNP 1552 1555 may may MD 1556 1565 influence influence VB 1566 1570 gene gene NN 1571 1581 expression expression NN 1582 1584 by by IN 1585 1603 ligand-independent ligand-independent JJ 1604 1614 mechanisms mechanism NNS 1614 1615 . . .