0	11	Interaction	interaction	NN
12	14	of	of	IN
15	21	sickle	sickle	JJ
22	34	erythrocytes	erythrocyte	NNS
35	39	with	with	IN
40	51	endothelial	endothelial	JJ
52	57	cells	cell	NNS
58	60	in	in	IN
61	64	the	the	DT
65	73	presence	presence	NN
74	76	of	of	IN
77	88	endothelial	endothelial	JJ
89	93	cell	cell	NN
94	105	conditioned	conditioned	JJ
106	112	medium	medium	NN
113	120	induces	induce	VBZ
121	128	oxidant	oxidant	JJ
129	135	stress	stress	NN
136	143	leading	lead	VBG
144	146	to	to	TO
147	163	transendothelial	transendothelial	JJ
164	173	migration	migration	NN
174	176	of	of	IN
177	186	monocytes	monocyte	NNS
186	187	.	.	.

189	192	The	the	DT
193	201	abnormal	abnormal	JJ
202	211	adherence	adherence	NN
212	214	of	of	IN
215	221	sickle	sickle	JJ
222	225	red	red	JJ
226	231	blood	blood	NN
232	237	cells	cell	NNS
238	239	(	(	(
239	241	SS	SS	NNP
242	245	RBC	RBC	NNP
245	246	)	)	)
247	249	to	to	TO
250	261	endothelial	endothelial	JJ
262	267	cells	cell	NNS
268	271	has	have	VBZ
272	276	been	be	VBN
277	284	thought	think	VBN
285	287	to	to	TO
288	298	contribute	contribute	VB
299	301	to	to	TO
302	310	vascular	vascular	JJ
311	320	occlusion	occlusion	NN
320	321	,	,	,
322	323	a	a	DT
324	329	major	major	JJ
330	335	cause	cause	NN
336	338	of	of	IN
339	348	morbidity	morbidity	NN
349	351	in	in	IN
352	358	sickle	sickle	JJ
359	363	cell	cell	NN
364	371	disease	disease	NN
372	373	(	(	(
373	376	SCD	SCD	NNP
376	377	)	)	)
377	378	.	.	.

379	381	We	we	PRP
382	392	determined	determine	VBD
393	400	whether	whether	IN
401	404	the	the	DT
405	416	interaction	interaction	NN
417	419	of	of	IN
420	422	SS	SS	NNP
423	426	RBC	RBC	NNP
427	431	with	with	IN
432	440	cultured	culture	VBN
441	452	endothelial	endothelial	JJ
453	458	cells	cell	NNS
459	466	induced	induce	VBD
467	475	cellular	cellular	JJ
476	483	oxidant	oxidant	JJ
484	490	stress	stress	NN
491	495	that	that	WDT
496	501	would	would	MD
502	511	culminate	culminate	VB
512	514	in	in	IN
515	525	expression	expression	NN
526	528	of	of	IN
529	533	cell	cell	NN
534	542	adhesion	adhesion	NN
543	552	molecules	molecule	NNS
553	554	(	(	(
554	558	CAMs	CAM	NNP
558	559	)	)	)
560	568	involved	involve	VBN
569	571	in	in	IN
572	575	the	the	DT
576	584	adhesion	adhesion	NN
585	588	and	and	CC
589	599	diapedesis	diapedesis	NN
600	602	of	of	IN
603	612	monocytes	monocyte	NNS
613	616	and	and	CC
617	620	the	the	DT
621	630	adherence	adherence	NN
631	633	of	of	IN
634	636	SS	SS	NNP
637	650	reticulocytes	reticulocyte	NNS
650	651	.	.	.

652	654	We	we	PRP
655	661	showed	show	VBD
662	666	that	that	IN
667	670	the	the	DT
671	682	interaction	interaction	NN
683	685	of	of	IN
686	688	SS	SS	NNP
689	692	RBC	RBC	NNP
693	695	at	at	IN
696	697	2	2	CD
697	698	%	%	NN
699	712	concentration	concentration	NN
713	715	in	in	IN
716	719	the	the	DT
720	728	presence	presence	NN
729	731	of	of	IN
732	741	multimers	multimer	NNS
742	744	of	of	IN
745	748	von	von	NNP
749	759	Willebrand	Willebrand	NNP
760	766	factor	factor	NN
767	768	(	(	(
768	771	vWf	vwf	NN
771	772	)	)	)
772	773	,	,	,
774	781	derived	derive	VBN
782	786	from	from	IN
787	798	endothelial	endothelial	JJ
799	811	cell-derived	cell-derived	JJ
812	823	conditioned	condition	VBN
824	830	medium	medium	NN
831	832	(	(	(
832	836	E-CM	E-CM	NNP
836	837	)	)	)
838	842	with	with	IN
843	851	cultured	culture	VBN
852	857	human	human	JJ
858	867	umbilical	umbilical	JJ
868	872	vein	vein	NN
873	884	endothelial	endothelial	JJ
885	890	cells	cell	NNS
891	892	(	(	(
892	897	HUVEC	HUVEC	NNP
897	898	)	)	)
898	899	,	,	,
900	908	resulted	result	VBD
909	911	in	in	IN
912	913	a	a	DT
914	922	fivefold	fivefold	RB
923	932	increased	increase	VBN
933	942	formation	formation	NN
943	945	of	of	IN
946	960	thiobarbituric	thiobarbituric	JJ
961	974	acid-reactive	acid-reactive	JJ
975	985	substances	substance	NNS
986	987	(	(	(
987	992	TBARS	TBARS	NNPS
992	993	)	)	)
994	997	and	and	CC
998	1008	activation	activation	NN
1009	1011	of	of	IN
1012	1015	the	the	DT
1016	1029	transcription	transcription	NN
1030	1036	factor	factor	NN
1037	1042	NF-kB	NF-kB	NNP
1042	1043	,	,	,
1044	1048	both	both	DT
1049	1059	indicators	indicator	NNS
1060	1062	of	of	IN
1063	1071	cellular	cellular	JJ
1072	1079	oxidant	oxidant	JJ
1080	1086	stress	stress	NN
1086	1087	.	.	.

1088	1094	Normal	normal	JJ
1095	1098	RBC	RBC	NNP
1099	1103	show	show	VBP
1104	1108	none	none	NN
1109	1111	of	of	IN
1112	1117	these	these	DT
1118	1127	phenomena	phenomenon	NNS
1127	1128	.	.	.

1129	1132	The	the	DT
1133	1140	oxidant	oxidant	JJ
1141	1155	stress-induced	stress-induced	JJ
1156	1165	signaling	signaling	NN
1166	1174	resulted	result	VBD
1175	1177	in	in	IN
1178	1180	an	an	DT
1181	1190	increased	increase	VBN
1191	1198	surface	surface	NN
1199	1209	expression	expression	NN
1210	1212	of	of	IN
1213	1214	a	a	DT
1215	1221	subset	subset	NN
1222	1224	of	of	IN
1225	1229	CAMs	CAM	NNP
1229	1230	,	,	,
1231	1237	ICAM-1	ICAM-1	NNP
1237	1238	,	,	,
1239	1249	E-selectin	E-selectin	NNP
1249	1250	,	,	,
1251	1254	and	and	CC
1255	1261	VCAM-1	vcam-1	NN
1262	1264	in	in	IN
1265	1270	HUVEC	HUVEC	NNP
1270	1271	.	.	.

1272	1275	The	the	DT
1276	1284	addition	addition	NN
1285	1287	of	of	IN
1288	1294	oxygen	oxygen	NN
1295	1302	radical	radical	NN
1303	1312	scavenger	scavenger	NN
1313	1320	enzymes	enzyme	NNS
1321	1322	(	(	(
1322	1330	catalase	catalase	NN
1330	1331	,	,	,
1332	1342	superoxide	superoxide	NN
1343	1352	dismutase	dismutase	NN
1352	1353	)	)	)
1354	1357	and	and	CC
1358	1369	antioxidant	antioxidant	NN
1370	1371	(	(	(
1371	1379	probucol	probucol	NN
1379	1380	)	)	)
1381	1390	inhibited	inhibit	VBD
1391	1396	these	these	DT
1397	1403	events	event	NNS
1403	1404	.	.	.

1405	1417	Additionally	additionally	RB
1417	1418	,	,	,
1419	1432	preincubation	preincubation	NN
1433	1435	of	of	IN
1436	1441	HUVEC	HUVEC	NNP
1442	1446	with	with	IN
1447	1448	a	a	DT
1449	1458	synthetic	synthetic	JJ
1459	1466	peptide	peptide	NN
1467	1478	Arg-Gly-Asp	Arg-Gly-Asp	NNP
1479	1480	(	(	(
1480	1483	RGD	RGD	NNP
1483	1484	)	)	)
1485	1489	that	that	WDT
1490	1498	prevents	prevent	VBZ
1499	1511	vWf-mediated	vwf-mediated	JJ
1512	1520	adhesion	adhesion	NN
1521	1523	of	of	IN
1524	1526	SS	SS	NNP
1527	1530	RBC	RBC	NNP
1531	1538	reduced	reduce	VBD
1539	1542	the	the	DT
1543	1550	surface	surface	NN
1551	1561	expression	expression	NN
1562	1564	of	of	IN
1565	1571	VCAM-1	VCAM-1	NNP
1572	1575	and	and	CC
1576	1581	NF-kB	NF-kB	NNP
1582	1592	activation	activation	NN
1592	1593	.	.	.

1594	1605	Furthermore	furthermore	RB
1605	1606	,	,	,
1607	1609	SS	SS	NNP
1610	1621	RBC-induced	rbc-induced	JJ
1622	1629	oxidant	oxidant	JJ
1630	1636	stress	stress	NN
1637	1645	resulted	result	VBD
1646	1648	in	in	IN
1649	1650	a	a	DT
1651	1658	twofold	twofold	JJ
1659	1667	increase	increase	NN
1668	1670	in	in	IN
1671	1674	the	the	DT
1675	1691	transendothelial	transendothelial	JJ
1692	1701	migration	migration	NN
1702	1704	of	of	IN
1705	1709	both	both	CC
1710	1723	monocyte-like	monocyte-like	JJ
1724	1729	HL-60	hl-60	NN
1730	1735	cells	cell	NNS
1736	1739	and	and	CC
1740	1745	human	human	JJ
1746	1756	peripheral	peripheral	JJ
1757	1762	blood	blood	NN
1763	1772	monocytes	monocyte	NNS
1772	1773	,	,	,
1774	1777	and	and	CC
1778	1791	approximately	approximately	RB
1792	1793	a	a	DT
1794	1801	sixfold	sixfold	JJ
1802	1810	increase	increase	NN
1811	1813	in	in	IN
1814	1834	platelet-endothelial	platelet-endothelial	JJ
1835	1839	cell	cell	NN
1840	1848	adhesion	adhesion	NN
1849	1859	molecule-1	molecule-1	NN
1860	1861	(	(	(
1861	1868	PECAM-1	pecam-1	NN
1868	1869	)	)	)
1870	1885	phosphorylation	phosphorylation	NN
1885	1886	,	,	,
1887	1891	each	each	DT
1892	1894	of	of	IN
1895	1900	which	which	WDT
1901	1904	was	be	VBD
1905	1912	blocked	block	VBN
1913	1915	by	by	IN
1916	1923	protein	protein	NN
1924	1930	kinase	kinase	NNP
1931	1932	C	C	NNP
1933	1942	inhibitor	inhibitor	NN
1943	1946	and	and	CC
1947	1959	antioxidants	antioxidant	NNS
1959	1960	.	.	.

1961	1966	These	these	DT
1967	1974	results	result	NNS
1975	1982	suggest	suggest	VBP
1983	1987	that	that	IN
1988	1991	the	the	DT
1992	2009	adherence/contact	adherence/contact	NN
2010	2012	of	of	IN
2013	2015	SS	SS	NNP
2016	2019	RBC	RBC	NNP
2020	2022	to	to	TO
2023	2034	endothelial	endothelial	JJ
2035	2040	cells	cell	NNS
2041	2043	in	in	IN
2044	2049	large	large	JJ
2050	2056	vessel	vessel	NN
2057	2060	can	can	MD
2061	2069	generate	generate	VB
2070	2078	enhanced	enhance	VBN
2079	2086	oxidant	oxidant	JJ
2087	2093	stress	stress	NN
2094	2101	leading	lead	VBG
2102	2104	to	to	TO
2105	2114	increased	increase	VBN
2115	2123	adhesion	adhesion	NN
2124	2127	and	and	CC
2128	2138	diapedesis	diapedesis	NN
2139	2141	of	of	IN
2142	2151	monocytes	monocyte	NNS
2151	2152	,	,	,
2153	2155	as	as	RB
2156	2160	well	well	RB
2161	2163	as	as	IN
2164	2174	heightened	heighten	VBN
2175	2184	adherence	adherence	NN
2185	2187	of	of	IN
2188	2190	SS	SS	NNP
2191	2204	reticulocytes	reticulocyte	NNS
2204	2205	,	,	,
2206	2216	indicating	indicate	VBG
2217	2221	that	that	IN
2222	2239	injury/activation	injury/activation	NN
2240	2242	of	of	IN
2243	2254	endothelium	endothelium	NN
2255	2258	can	can	MD
2259	2269	contribute	contribute	VB
2270	2272	to	to	TO
2273	2287	vaso-occlusion	vaso-occlusion	NN
2288	2290	in	in	IN
2291	2294	SCD	SCD	NNP
2294	2295	.	.	.