0 11 Granulocyte Granulocyte NNP 12 30 colony-stimulating colony-stimulating JJ 31 37 factor factor NN 38 47 activates activate VBZ 48 49 a a DT 50 56 72-kDa 72-kda JJ 57 64 isoform isoform NN 65 67 of of IN 68 73 STAT3 stat3 NN 74 76 in in IN 77 82 human human JJ 83 94 neutrophils neutrophil NNS 94 95 . . . 97 108 Granulocyte Granulocyte NNP 109 127 colony-stimulating colony-stimulating JJ 128 134 factor factor NN 135 136 ( ( ( 136 141 G-CSF G-CSF NNP 141 142 ) ) ) 143 152 signaling signaling NN 153 161 involves involve VBZ 162 172 activation activation NN 173 175 of of IN 176 181 STATs STAT NNP 181 182 , , , 183 191 proteins protein NNS 192 196 that that WDT 197 202 serve serve VBP 203 206 the the DT 207 211 dual dual JJ 212 220 function function NN 221 223 of of IN 224 230 signal signal NN 231 243 transduction transduction NN 244 247 and and CC 248 258 activation activation NN 259 261 of of IN 262 275 transcription transcription NN 275 276 . . . 277 279 We we PRP 280 290 previously previously RB 291 303 demonstrated demonstrate VBD 304 308 that that IN 309 314 G-CSF G-CSF NNP 315 324 activated activate VBD 325 326 a a DT 327 335 distinct distinct JJ 336 346 Stat3-like stat3-like JJ 347 354 protein protein NN 355 357 in in IN 358 366 immature immature JJ 367 370 and and CC 371 377 mature mature JJ 378 384 normal normal JJ 385 392 myeloid myeloid JJ 393 398 cells cell NNS 398 399 , , , 400 405 StatG StatG NNP 405 406 . . . 407 412 StatG StatG NNP 413 415 in in IN 416 422 normal normal JJ 423 431 immature immature JJ 432 437 human human JJ 438 445 myeloid myeloid JJ 446 451 cells cell NNS 451 452 , , , 453 457 i.e. i.e. FW 458 463 adult adult JJ 464 469 CD34+ cd34+ JJ 470 474 bone bone NN 475 481 marrow marrow NN 482 487 cells cell NNS 487 488 , , , 489 492 was be VBD 493 501 composed compose VBN 502 504 of of IN 505 514 Stat3beta Stat3beta NNP 514 515 . . . 516 520 This this DT 521 534 investigation investigation NN 535 538 was be VBD 539 549 undertaken undertake VBN 550 552 to to TO 553 562 determine determine VB 563 566 the the DT 567 578 composition composition NN 579 581 of of IN 582 587 StatG statg NN 588 590 in in IN 591 597 mature mature JJ 598 604 normal normal JJ 605 610 human human JJ 611 618 myeloid myeloid JJ 619 624 cells cell NNS 624 625 , , , 626 630 i.e. i.e. FW 631 648 polymorphonuclear polymorphonuclear JJ 649 661 neutrophilic neutrophilic JJ 662 674 granulocytes granulocyte NNS 675 676 ( ( ( 676 679 PMN PMN NNP 679 680 ) ) ) 680 681 . . . 682 687 These these DT 688 695 studies study NNS 696 704 revealed reveal VBD 705 709 that that IN 710 713 the the DT 714 719 major major JJ 720 727 protein protein NN 728 730 in in IN 731 739 extracts extract NNS 740 742 of of IN 743 746 PMN PMN NNP 747 756 activated activate VBN 757 759 by by IN 760 765 G-CSF G-CSF NNP 766 768 to to TO 769 773 bind bind VB 774 777 the the DT 778 791 high-affinity high-affinity JJ 792 807 serum-inducible serum-inducible JJ 808 815 element element NN 816 817 ( ( ( 817 821 hSIE hSIE NNP 821 822 ) ) ) 823 825 is be VBZ 826 827 a a DT 828 834 72-kDa 72-kda JJ 835 842 protein protein NN 843 847 that that WDT 848 860 cross-reacts cross-react VBZ 861 865 with with IN 866 871 Stat3 stat3 NN 872 882 monoclonal monoclonal JJ 883 891 antibody antibody NN 891 892 , , , 893 898 which which WDT 899 901 we we PRP 902 906 have have VBP 907 917 designated designate VBN 918 928 Stat3gamma Stat3gamma NNP 928 929 . . . 930 940 Stat3gamma Stat3gamma NNP 941 943 is be VBZ 944 951 derived derive VBN 952 956 from from IN 957 967 Stat3alpha Stat3alpha NNP 968 970 by by IN 971 978 limited limited JJ 979 990 proteolysis proteolysis NN 991 994 and and CC 995 1000 lacks lack VBZ 1001 1004 the the DT 1005 1022 carboxyl-terminal carboxyl-terminal JJ 1023 1030 portion portion NN 1031 1033 of of IN 1034 1044 Stat3alpha Stat3alpha NNP 1044 1045 . . . 1046 1053 Because because IN 1054 1058 this this DT 1059 1065 region region NN 1066 1068 of of IN 1069 1079 Stat3alpha Stat3alpha NNP 1080 1082 is be VBZ 1083 1091 involved involve VBN 1092 1094 in in IN 1095 1110 transcriptional transcriptional JJ 1111 1121 activation activation NN 1121 1122 , , , 1123 1126 our our PRP$ 1127 1135 findings finding NNS 1136 1143 suggest suggest VBP 1144 1147 the the DT 1148 1159 possibility possibility NN 1160 1164 that that IN 1165 1175 Stat3gamma Stat3gamma NNP 1176 1179 may may MD 1180 1182 be be VB 1183 1200 transcriptionally transcriptionally RB 1201 1209 inactive inactive JJ 1210 1213 and and CC 1214 1217 may may MD 1218 1225 compete compete VB 1226 1230 with with IN 1231 1241 Stat3alpha Stat3alpha NNP 1242 1245 for for IN 1246 1251 Stat3 stat3 NN 1252 1259 binding binding NN 1260 1265 sites site NNS 1266 1268 in in IN 1269 1274 these these DT 1275 1285 terminally terminally RB 1286 1300 differentiated differentiate VBN 1301 1308 myeloid myeloid JJ 1309 1314 cells cell NNS 1314 1315 . . .