0 7 Cloning cloning NN 8 10 of of IN 11 25 ARE-containing are-containing JJ 26 31 genes gene NNS 32 34 by by IN 35 52 AU-motif-directed au-motif-directed JJ 53 60 display display NN 60 61 . . . 63 64 A a DT 65 74 procedure procedure NN 75 83 suitable suitable JJ 84 87 for for IN 88 95 cloning clone VBG 96 102 labile labile JJ 103 108 mRNAs mrna NNS 109 113 that that WDT 114 121 contain contain VBP 122 124 AU AU NNP 125 131 motifs motif NNS 132 134 is be VBZ 135 144 presented present VBN 145 146 ( ( ( 146 151 AU-DD AU-DD NNP 151 152 ) ) ) 152 153 . . . 154 159 These these DT 160 166 motifs motif NNS 167 170 are be VBP 171 181 regulatory regulatory JJ 182 191 sequences sequence NNS 192 198 within within IN 199 202 the the DT 203 212 so-called so-called JJ 213 220 AU-rich au-rich JJ 221 229 elements element NNS 230 231 ( ( ( 231 235 AREs ARE NNP 235 236 ) ) ) 237 242 often often RB 243 248 found find VBN 249 251 in in IN 252 253 3 3 CD 253 254 ’ ' SYM 255 267 untranslated untranslated JJ 268 275 regions region NNS 276 278 of of IN 279 284 genes gene NNS 285 289 such such JJ 290 292 as as IN 293 302 cytokines cytokine NNS 302 303 , , , 304 319 proto-oncogenes proto-oncogene NNS 319 320 , , , 321 324 and and CC 325 338 transcription transcription NN 339 346 factors factor NNS 346 347 . . . 348 353 AU-DD AU-DD NNP 354 356 is be VBZ 357 359 an an DT 360 377 AU-motif-directed au-motif-directed JJ 378 390 differential differential JJ 391 398 display display NN 399 403 that that WDT 404 411 permits permit VBZ 412 415 the the DT 416 430 identification identification NN 431 433 of of IN 434 448 ARE-containing are-containing JJ 449 454 genes gene NNS 455 469 differentially differentially RB 470 479 expressed express VBN 480 485 after after IN 486 490 cell cell NN 491 501 activation activation NN 501 502 . . . 503 505 It it PRP 506 509 has have VBZ 510 514 been be VBN 515 522 applied apply VBN 523 525 to to TO 526 536 peripheral peripheral JJ 537 542 blood blood NN 543 552 monocytes monocyte NNS 553 556 and and CC 557 558 a a DT 559 560 T t NN 561 565 cell cell NN 566 571 clone clone NN 572 574 to to TO 575 582 isolate isolate VB 583 585 59 59 CD 586 590 cDNA cdna NN 591 600 fragments fragment NNS 601 611 associated associate VBN 612 614 to to TO 615 625 activation activation NN 625 626 . . . 627 635 Fourteen fourteen CD 636 643 percent percent NN 644 646 of of IN 647 655 isolated isolate VBN 656 665 fragments fragment NNS 666 672 belong belong VBP 673 675 to to TO 676 683 already already RB 684 689 known known JJ 690 695 genes gene NNS 696 700 that that WDT 701 710 certainly certainly RB 711 714 are be VBP 715 724 cytokines cytokine NNS 725 728 and and CC 729 755 transduction/transcription transduction/transcription NN 756 763 factors factor NNS 763 764 . . . 765 768 The the DT 769 778 remaining remain VBG 779 781 86 86 CD 781 782 % % NN 783 793 correspond correspond VBP 794 796 to to TO 797 804 unknown unknown JJ 805 810 genes gene NNS 811 813 of of IN 814 819 which which WDT 820 822 92 92 CD 822 823 % % NN 824 828 have have VBP 829 833 been be VBN 834 843 confirmed confirm VBN 844 846 to to TO 847 849 be be VB 850 864 differentially differentially RB 865 874 expressed express VBN 874 875 . . . 876 881 These these DT 882 886 data datum NNS 887 898 demonstrate demonstrate VBP 899 902 the the DT 903 913 efficiency efficiency NN 914 916 of of IN 917 920 the the DT 921 927 system system NN 928 931 and and CC 932 939 support support VBP 940 943 the the DT 944 950 notion notion NN 951 955 that that IN 956 964 numerous numerous JJ 965 970 genes gene NNS 971 978 falling fall VBG 979 983 into into IN 984 989 those those DT 990 1000 categories category NNS 1001 1007 remain remain VBP 1008 1020 unidentified unidentified JJ 1021 1024 and and CC 1025 1029 that that IN 1030 1034 they they PRP 1035 1038 can can MD 1039 1041 be be VB 1042 1048 cloned clone VBN 1049 1051 by by IN 1052 1056 this this DT 1057 1063 method method NN 1063 1064 . . . 1065 1074 Copyright Copyright NNP 1075 1079 1998 1998 CD 1080 1088 Academic Academic NNP 1089 1094 Press Press NNP 1094 1095 . . .