0 14 Interleukin-12 interleukin-12 NN 15 25 expression expression NN 26 28 in in IN 29 30 B B NNP 31 36 cells cell NNS 37 39 by by IN 40 54 transformation transformation NN 55 59 with with IN 60 72 Epstein-Barr epstein-barr JJ 73 78 virus virus NN 78 79 . . . 81 89 Although although IN 90 101 interleukin interleukin NN 102 103 ( ( ( 103 109 IL)-12 il)-12 NN 110 113 was be VBD 114 124 originally originally RB 125 133 purified purify VBN 134 138 from from IN 139 141 an an DT 142 154 Epstein-Barr epstein-barr JJ 155 156 ( ( ( 156 172 EBV)-transformed ebv)-transformed JJ 173 174 B B NNP 175 179 cell cell NN 180 184 line line NN 185 188 and and CC 189 192 the the DT 193 197 high high JJ 198 209 correlation correlation NN 210 212 of of IN 213 216 EBV EBV NNP 217 226 infection infection NN 227 230 and and CC 231 236 IL-12 il-12 NN 237 247 expression expression NN 248 251 has have VBZ 252 256 been be VBN 257 266 suggested suggest VBN 266 267 , , , 268 270 no no DT 271 276 study study NN 277 280 has have VBZ 281 289 reported report VBN 290 297 whether whether IN 298 301 EBV EBV NNP 302 311 infection infection NN 312 314 is be VBZ 315 323 directly directly RB 324 330 linked link VBN 331 333 to to TO 334 339 IL-12 il-12 NN 340 350 expression expression NN 350 351 . . . 352 354 To to TO 355 362 address address VB 363 367 this this DT 368 373 issue issue NN 373 374 , , , 375 377 we we PRP 378 382 have have VBP 383 395 investigated investigate VBN 396 401 IL-12 il-12 NN 402 412 expression expression NN 413 415 in in IN 416 417 B B NNP 418 423 cells cell NNS 424 430 during during IN 431 433 in in FW 434 439 vitro vitro FW 440 454 transformation transformation NN 455 459 with with IN 460 463 EBV EBV NNP 463 464 . . . 465 470 Human human JJ 471 481 peripheral peripheral JJ 482 483 B B NNP 484 489 cells cell NNS 490 496 became become VBD 497 504 capable capable JJ 505 507 of of IN 508 522 constitutively constitutively RB 523 532 producing produce VBG 533 536 p40 p40 NN 537 539 by by IN 540 542 in in FW 543 548 vitro vitro FW 549 563 transformation transformation NN 564 568 with with IN 569 572 EBV EBV NNP 572 573 , , , 574 584 coincident coincident JJ 585 589 with with IN 590 593 the the DT 594 604 expression expression NN 605 607 of of IN 608 614 latent latent JJ 615 623 membrane membrane NN 624 631 protein protein NN 632 633 1 1 CD 634 635 ( ( ( 635 639 LMP1 lmp1 NN 639 640 ) ) ) 641 643 of of IN 644 647 EBV EBV NNP 647 648 . . . 649 654 These these DT 655 656 B B NNP 657 662 cells cell NNS 663 672 expressed express VBD 673 676 p40 p40 NN 677 680 and and CC 681 684 p35 p35 NN 685 689 mRNA mrna NN 689 690 , , , 691 694 and and CC 695 702 phorbol phorbol NN 703 712 myristate myristate NN 713 720 acetate acetate NN 721 722 ( ( ( 722 725 PMA PMA NNP 725 726 ) ) ) 727 738 stimulation stimulation NN 739 747 strongly strongly RB 748 756 enhanced enhance VBD 757 760 p40 p40 NN 761 764 and and CC 765 768 p70 p70 NN 769 779 production production NN 779 780 . . . 781 792 Furthermore furthermore RB 792 793 , , , 794 806 transfection transfection NN 807 811 with with IN 812 816 LMP1 lmp1 NN 817 827 expression expression NN 828 834 vector vector NN 835 839 into into IN 840 841 a a DT 842 847 human human JJ 848 849 B B NNP 850 858 lymphoma lymphoma NN 859 863 cell cell NN 864 868 line line NN 868 869 , , , 870 875 Daudi Daudi NNP 875 876 , , , 877 880 led lead VBD 881 883 to to TO 884 887 p40 p40 NN 888 898 production production NN 899 903 with with IN 904 911 nuclear nuclear JJ 912 918 factor factor NN 919 920 ( ( ( 920 930 NF)-kappaB NF)-kappaB NNP 931 941 activation activation NN 941 942 . . . 943 948 These these DT 949 956 results result NNS 957 964 suggest suggest VBP 965 969 that that IN 970 984 transformation transformation NN 985 987 of of IN 988 995 primary primary JJ 996 997 B B NNP 998 1003 cells cell NNS 1004 1008 with with IN 1009 1012 EBV EBV NNP 1013 1020 induces induce VBZ 1021 1026 IL-12 il-12 NN 1027 1037 expression expression NN 1038 1049 potentially potentially RB 1050 1057 through through IN 1058 1062 LMP1 lmp1 NN 1063 1073 expression expression NN 1073 1074 . . . 1075 1084 Copyright Copyright NNP 1085 1089 1998 1998 CD 1090 1098 Academic Academic NNP 1099 1104 Press Press NNP 1104 1105 . . .