0 9 Molecular molecular JJ 10 17 cloning cloning NN 18 20 of of IN 21 29 FKHRL1P2 FKHRL1P2 NNP 29 30 , , , 31 32 a a DT 33 39 member member NN 40 42 of of IN 43 46 the the DT 47 62 developmentally developmentally RB 63 72 regulated regulate VBN 73 77 fork fork NN 78 82 head head NN 83 89 domain domain NN 90 103 transcription transcription NN 104 110 factor factor NN 111 117 family family NN 117 118 . . . 120 124 Here here RB 125 127 we we PRP 128 134 report report VBP 135 138 the the DT 139 149 expression expression NN 150 152 of of IN 153 154 a a DT 155 159 fork fork NN 160 164 head head NN 165 171 domain domain NN 172 179 protein protein NN 180 182 in in IN 183 188 human human JJ 189 190 T t NN 191 197 helper helper NN 198 203 cells cell NNS 203 204 . . . 205 207 We we PRP 208 214 cloned clone VBD 215 218 and and CC 219 232 characterized characterize VBD 233 234 a a DT 235 239 fork fork NN 240 244 head head NN 245 249 cDNA cdna NN 250 254 from from IN 255 260 human human JJ 261 262 T t NN 263 269 helper helper NN 270 274 cell cell NN 275 279 mRNA mrna NN 280 285 using use VBG 286 298 differential differential JJ 299 306 display display NN 307 313 RT-PCR rt-pcr NN 313 314 . . . 315 318 The the DT 319 323 cDNA cDNA NNP 324 332 contains contain VBZ 333 334 a a DT 335 349 546-nucleotide 546-nucleotide JJ 350 351 ( ( ( 351 353 nt nt NN 353 354 ) ) ) 355 359 open open JJ 360 367 reading reading NN 368 373 frame frame NN 374 375 ( ( ( 375 378 ORF ORF NNP 378 379 ) ) ) 380 384 that that WDT 385 390 codes cod VBZ 391 394 for for IN 395 398 the the DT 399 416 carboxyl-terminal carboxyl-terminal JJ 417 420 180 180 CD 421 426 amino amino NN 427 432 acids acid NNS 433 434 ( ( ( 434 436 aa aa NN 436 437 ) ) ) 438 440 of of IN 441 444 the the DT 445 453 recently recently RB 454 464 identified identify VBN 465 471 fkhrl1 fkhrl1 NN 472 476 gene gene NN 476 477 . . . 478 482 This this DT 483 486 ORF orf NN 487 491 does do VBZ 492 495 not not RB 496 503 contain contain VB 504 507 the the DT 508 522 characteristic characteristic JJ 523 534 DNA-binding dna-binding JJ 535 541 domain domain NN 542 547 found find VBN 548 550 in in IN 551 558 members member NNS 559 561 of of IN 562 565 the the DT 566 574 forkhead forkhead NN 575 582 protein protein NN 583 589 family family NN 589 590 . . . 591 599 In-vitro in-vitro FW 600 625 transcription/translation transcription/translation NN 626 628 of of IN 629 633 this this DT 634 638 cDNA cDNA NNP 639 648 expressed express VBD 649 650 a a DT 651 658 protein protein NN 659 661 of of IN 662 675 approximately approximately RB 676 678 20 20 CD 679 682 kDa kda NN 682 683 . . . 684 686 We we PRP 687 691 have have VBP 692 701 generated generate VBN 702 712 antibodies antibody NNS 713 717 that that WDT 718 730 specifically specifically RB 731 749 immunoprecipitated immunoprecipitate VBD 750 753 the the DT 754 773 in-vitro-translated in-vitro-translated JJ 774 780 20-kDa 20-kda JJ 781 788 protein protein NN 788 789 . . . 790 794 This this DT 795 803 antibody antibody NN 804 808 also also RB 809 819 recognizes recognize VBZ 820 822 in in IN 823 828 human human JJ 829 830 T t NN 831 842 lymphocytes lymphocyte NNS 843 844 a a DT 845 851 70-kDa 70-kda JJ 852 859 protein protein NN 860 873 corresponding correspond VBG 874 876 in in IN 877 881 size size NN 882 884 to to TO 885 889 that that DT 890 899 predicted predict VBN 900 903 for for IN 904 907 the the DT 908 914 fkhrl1 fkhrl1 NN 915 919 gene gene NN 920 927 product product NN 927 928 . . . 929 932 The the DT 933 937 mRNA mrna NN 938 944 levels level NNS 945 948 for for IN 949 955 fkhrl1 fkhrl1 NN 956 958 is be VBZ 959 967 elevated elevate VBN 968 970 in in IN 971 972 T t NN 973 987 helper-induced helper-induced JJ 988 999 lymphocytes lymphocyte NNS 1000 1002 in in IN 1003 1013 comparison comparison NN 1014 1016 to to TO 1017 1031 PHA-stimulated pha-stimulated JJ 1032 1033 T t NN 1034 1045 lymphocytes lymphocyte NNS 1045 1046 . . . 1047 1054 Further further JJ 1055 1071 characterization characterization NN 1072 1074 of of IN 1075 1081 FKHRL1 FKHRL1 NNP 1082 1085 and and CC 1086 1089 its its PRP$ 1090 1097 related related JJ 1098 1104 family family NN 1105 1112 members member NNS 1113 1119 should should MD 1120 1124 shed shed VB 1125 1130 light light NN 1131 1133 on on IN 1134 1137 the the DT 1138 1153 transcriptional transcriptional JJ 1154 1164 mechanisms mechanism NNS 1165 1167 of of IN 1168 1172 this this DT 1173 1177 fork fork NN 1178 1182 head head NN 1183 1187 gene gene NN 1188 1197 subfamily subfamily NN 1198 1201 and and CC 1202 1207 their their PRP$ 1208 1212 role role NN 1213 1215 in in IN 1216 1217 T t NN 1218 1224 helper helper NN 1225 1229 cell cell NN 1230 1245 differentiation differentiation NN 1246 1249 and and CC 1250 1260 regulation regulation NN 1261 1263 of of IN 1264 1268 cell cell NN 1269 1275 growth growth NN 1275 1276 . . .