0 12 Differential differential JJ 13 21 monocyte monocyte NN 22 30 adhesion adhesion NN 31 34 and and CC 35 43 adhesion adhesion NN 44 52 molecule molecule NN 53 63 expression expression NN 64 66 in in IN 67 73 venous venous JJ 74 77 and and CC 78 86 arterial arterial JJ 87 98 endothelial endothelial JJ 99 104 cells cell NNS 104 105 . . . 107 109 We we PRP 110 118 compared compare VBD 119 124 U-937 u-937 NN 125 129 cell cell NN 130 138 adhesion adhesion NN 139 142 and and CC 143 151 adhesion adhesion NN 152 160 molecule molecule NN 161 171 expression expression NN 172 174 in in IN 175 180 human human JJ 181 190 umbilical umbilical JJ 191 197 venous venous JJ 198 199 ( ( ( 199 205 HUVECs huvec NNS 205 206 ) ) ) 207 210 and and CC 211 219 arterial arterial JJ 220 221 ( ( ( 221 227 HUAECs HUAEC NNP 227 228 ) ) ) 229 240 endothelial endothelial JJ 241 246 cells cell NNS 247 254 exposed expose VBN 255 257 to to TO 258 263 tumor tumor NN 264 272 necrosis necrosis NN 273 279 factor factor NN 280 281 ( ( ( 281 284 TNF TNF NNP 284 285 ) ) ) 285 286 , , , 287 300 interleukin-1 interleukin-1 NN 300 301 , , , 302 305 and and CC 306 324 lipopolysaccharide lipopolysaccharide NN 325 326 ( ( ( 326 329 LPS LPS NNP 329 330 ) ) ) 330 331 . . . 332 335 TNF TNF NNP 336 339 and and CC 340 343 LPS lps NN 344 354 stimulated stimulate VBD 355 363 vascular vascular JJ 364 368 cell cell NN 369 377 adhesion adhesion NN 378 386 molecule molecule NN 387 388 ( ( ( 388 395 VCAM)-1 vcam)-1 NN 396 403 surface surface NN 404 414 expression expression NN 415 418 and and CC 419 427 adhesion adhesion NN 428 430 of of IN 431 436 U-937 u-937 NN 437 450 monocyte-like monocyte-like JJ 451 456 cells cell NNS 457 459 to to TO 460 466 HUVECs huvec NNS 467 470 but but CC 471 474 not not RB 475 477 to to TO 478 484 HUAECs huaec NNS 484 485 . . . 486 494 Antibody antibody NN 495 502 studies study NNS 503 515 demonstrated demonstrate VBD 516 520 that that IN 521 523 in in IN 524 530 HUVECs huvec NNS 531 533 at at IN 534 539 least least JJS 540 542 75 75 CD 542 543 % % NN 544 546 of of IN 547 550 the the DT 551 559 adhesion adhesion NN 560 568 response response NN 569 571 is be VBZ 572 578 VCAM-1 vcam-1 NN 579 587 mediated mediate VBN 587 588 . . . 589 602 Interleukin-1 interleukin-1 NN 603 613 stimulated stimulate VBD 614 619 U-937 u-937 NN 620 624 cell cell NN 625 633 adhesion adhesion NN 634 636 to to TO 637 640 and and CC 641 647 VCAM-1 vcam-1 NN 648 655 surface surface NN 656 666 expression expression NN 667 669 in in IN 670 674 both both CC 675 681 HUVECs HUVEC NNPS 682 685 and and CC 686 692 HUAECs HUAEC NNPS 692 693 . . . 694 720 Pyrrolidinedithiocarbamate Pyrrolidinedithiocarbamate NNP 721 724 and and CC 725 728 the the DT 729 739 proteasome proteasome NN 740 749 inhibitor inhibitor NN 750 756 MG-132 mg-132 NN 757 764 blocked block VBD 765 769 TNF- tnf- NN 770 773 and and CC 774 788 LPS-stimulated lps-stimulated JJ 789 794 U-937 u-937 NN 795 799 cell cell NN 800 808 adhesion adhesion NN 809 811 to to TO 812 818 HUVECs huvec NNS 818 819 . . . 820 825 These these DT 826 832 agents agent NNS 833 837 also also RB 838 851 significantly significantly RB 852 861 decreased decrease VBD 862 866 TNF- tnf- NN 867 870 and and CC 871 885 LPS-stimulated lps-stimulated JJ 886 895 increases increase NNS 896 898 in in IN 899 904 HUVEC HUVEC NNP 905 912 surface surface NN 913 919 VCAM-1 vcam-1 NN 919 920 . . . 921 924 TNF TNF NNP 925 934 increased increase VBD 935 941 VCAM-1 vcam-1 NN 942 949 protein protein NN 950 953 and and CC 954 958 mRNA mrna NN 959 961 in in IN 962 968 HUVECs HUVEC NNP 969 973 that that WDT 974 977 was be VBD 978 985 blocked block VBN 986 988 by by IN 989 1015 pyrrolidinedithiocarbamate pyrrolidinedithiocarbamate NN 1015 1016 . . . 1017 1024 However however RB 1024 1025 , , , 1026 1033 neither neither CC 1034 1037 TNF TNF NNP 1038 1040 or or CC 1041 1044 LPS lps NN 1045 1055 stimulated stimulate VBD 1056 1062 VCAM-1 vcam-1 NN 1063 1073 expression expression NN 1074 1076 in in IN 1077 1083 HUAECs HUAEC NNP 1083 1084 . . . 1085 1088 TNF TNF NNP 1089 1099 stimulated stimulate VBD 1100 1110 expression expression NN 1111 1113 of of IN 1114 1118 both both CC 1119 1132 intercellular intercellular JJ 1133 1141 adhesion adhesion NN 1142 1152 molecule-1 molecule-1 NN 1153 1156 and and CC 1157 1167 E-selectin E-selectin NNP 1168 1170 in in IN 1171 1177 HUVECs HUVEC NNP 1177 1178 , , , 1179 1182 but but CC 1183 1185 in in IN 1186 1192 HUAECs HUAEC NNP 1192 1193 , , , 1194 1198 only only RB 1199 1212 intercellular intercellular JJ 1213 1221 adhesion adhesion NN 1222 1232 molecule-1 molecule-1 NN 1233 1236 was be VBD 1237 1246 increased increase VBN 1246 1247 . . . 1248 1263 Electrophoretic electrophoretic JJ 1264 1272 mobility mobility NN 1273 1278 shift shift NN 1279 1285 assays assay NNS 1286 1298 demonstrated demonstrate VBD 1299 1301 no no DT 1302 1312 difference difference NN 1313 1315 in in IN 1316 1319 the the DT 1320 1327 pattern pattern NN 1328 1330 of of IN 1331 1345 TNF-stimulated tnf-stimulated JJ 1346 1353 nuclear nuclear JJ 1354 1367 factor-kappaB factor-kappab NN 1368 1378 activation activation NN 1379 1386 between between IN 1387 1393 HUVECs HUVEC NNPS 1394 1397 and and CC 1398 1404 HUAECs HUAEC NNPS 1404 1405 . . . 1406 1411 These these DT 1412 1419 studies study NNS 1420 1431 demonstrate demonstrate VBP 1432 1433 a a DT 1434 1439 novel novel JJ 1440 1443 and and CC 1444 1452 striking striking JJ 1453 1466 insensitivity insensitivity NN 1467 1469 of of IN 1470 1478 arterial arterial JJ 1479 1490 endothelium endothelium NN 1491 1493 to to TO 1494 1497 the the DT 1498 1505 effects effect NNS 1506 1508 of of IN 1509 1512 TNF TNF NNP 1513 1516 and and CC 1517 1520 LPS LPS NNP 1521 1524 and and CC 1525 1533 indicate indicate VBP 1534 1535 a a DT 1536 1548 dissociation dissociation NN 1549 1556 between between IN 1557 1560 the the DT 1561 1568 ability ability NN 1569 1571 of of IN 1572 1578 HUAECs huaec NNS 1579 1581 to to TO 1582 1592 upregulate upregulate VB 1593 1600 nuclear nuclear JJ 1601 1614 factor-kappaB factor-kappab NN 1615 1618 and and CC 1619 1625 VCAM-1 VCAM-1 NNP 1625 1626 . . .