0 11 Involvement involvement NN 12 14 of of IN 15 32 mitogen-activated mitogen-activated JJ 33 40 protein protein NN 41 47 kinase kinase NNP 48 56 pathways pathway NNS 57 59 in in IN 60 73 interleukin-8 interleukin-8 NN 74 84 production production NN 85 87 by by IN 88 93 human human JJ 94 103 monocytes monocyte NNS 104 107 and and CC 108 125 polymorphonuclear polymorphonuclear JJ 126 131 cells cell NNS 132 142 stimulated stimulate VBN 143 147 with with IN 148 166 lipopolysaccharide lipopolysaccharide NN 167 169 or or CC 170 180 Mycoplasma Mycoplasma NNP 181 191 fermentans fermentan NNS 192 200 membrane membrane NN 201 213 lipoproteins lipoprotein NNS 213 214 . . . 216 229 Interleukin-8 Interleukin-8 NNP 230 231 ( ( ( 231 235 IL-8 il-8 NN 235 236 ) ) ) 237 239 is be VBZ 240 241 a a DT 242 251 chemokine chemokine NN 252 256 that that WDT 257 264 belongs belong VBZ 265 267 to to TO 268 271 the the DT 272 287 alpha-chemokine alpha-chemokine NN 288 290 or or CC 291 294 CXC CXC NNP 295 304 subfamily subfamily NN 305 308 and and CC 309 311 is be VBZ 312 320 produced produce VBN 321 323 by by IN 324 325 a a DT 326 330 wide wide JJ 331 338 variety variety NN 339 341 of of IN 342 347 human human JJ 348 353 cells cell NNS 353 354 , , , 355 364 including include VBG 365 374 monocytes monocyte NNS 375 378 and and CC 379 396 polymorphonuclear polymorphonuclear JJ 397 402 cells cell NNS 403 404 ( ( ( 404 407 PMN PMN NNP 407 408 ) ) ) 408 409 . . . 410 414 IL-8 il-8 NN 415 417 is be VBZ 418 426 secreted secrete VBN 427 429 in in IN 430 438 response response NN 439 441 to to TO 442 454 inflammatory inflammatory JJ 455 462 stimuli stimulus NNS 462 463 , , , 464 471 notably notably RB 472 481 bacterial bacterial JJ 482 490 products product NNS 491 495 such such JJ 496 498 as as IN 499 517 lipopolysaccharide lipopolysaccharide NN 518 519 ( ( ( 519 522 LPS LPS NNP 522 523 ) ) ) 523 524 , , , 525 528 but but CC 529 535 little little JJ 536 538 is be VBZ 539 544 known know VBN 545 550 about about IN 551 554 the the DT 555 565 mechanisms mechanism NNS 566 568 by by IN 569 574 which which WDT 575 580 these these DT 581 587 agents agent NNS 588 595 mediate mediate VBP 596 600 IL-8 il-8 NN 601 610 induction induction NN 610 611 . . . 612 614 In in IN 615 619 this this DT 620 626 report report NN 626 627 , , , 628 630 we we PRP 631 635 show show VBP 636 640 that that IN 641 651 Mycoplasma Mycoplasma NNP 652 662 fermentans fermentan NNS 663 679 lipid-associated lipid-associated JJ 680 688 membrane membrane NN 689 697 proteins protein NNS 698 699 ( ( ( 699 704 LAMPf LAMPf NNP 704 705 ) ) ) 706 712 induce induce VBP 713 716 the the DT 717 727 production production NN 728 730 of of IN 731 735 high high JJ 736 742 levels level NNS 743 745 of of IN 746 750 IL-8 il-8 NN 751 753 by by IN 754 759 THP-1 thp-1 NN 760 761 ( ( ( 761 766 human human JJ 767 775 monocyte monocyte NN 775 776 ) ) ) 777 782 cells cell NNS 783 786 and and CC 787 790 PMN pmn NN 791 793 at at IN 794 797 the the DT 798 802 same same JJ 803 809 extent extent NN 810 812 as as IN 813 816 LPS LPS NNP 816 817 . . . 818 820 It it PRP 821 824 was be VBD 825 835 previously previously RB 836 848 demonstrated demonstrate VBN 849 853 that that IN 854 865 stimulation stimulation NN 866 868 of of IN 869 878 monocytic monocytic JJ 879 884 cells cell NNS 885 889 with with IN 890 896 either either CC 897 900 LPS LPS NNP 901 903 or or CC 904 909 LAMPf LAMPf NNP 910 913 led lead VBD 914 916 to to TO 917 918 a a DT 919 925 series series NN 926 928 of of IN 929 935 common common JJ 936 946 downstream downstream JJ 947 956 signaling signaling NN 957 963 events event NNS 963 964 , , , 965 974 including include VBG 975 978 the the DT 979 989 activation activation NN 990 992 of of IN 993 1000 protein protein NN 1001 1009 tyrosine tyrosine NN 1010 1016 kinase kinase NNP 1017 1020 and and CC 1021 1023 of of IN 1024 1041 mitogen-activated mitogen-activated JJ 1042 1049 protein protein NN 1050 1056 kinase kinase NNP 1057 1065 cascades cascade NNS 1065 1066 . . . 1067 1069 By by IN 1070 1075 using use VBG 1076 1084 PD-98059 PD-98059 NNP 1085 1088 and and CC 1089 1097 SB203580 SB203580 NNP 1097 1098 , , , 1099 1102 two two CD 1103 1109 potent potent JJ 1110 1113 and and CC 1114 1123 selective selective JJ 1124 1134 inhibitors inhibitor NNS 1135 1137 of of IN 1138 1142 MEK1 MEK1 NNP 1143 1144 ( ( ( 1144 1145 a a DT 1146 1152 kinase kinase NNP 1153 1161 upstream upstream RB 1162 1164 of of IN 1165 1171 ERK1/2 ERK1/2 NNP 1171 1172 ) ) ) 1173 1176 and and CC 1177 1180 p38 p38 NN 1180 1181 , , , 1182 1194 respectively respectively RB 1194 1195 , , , 1196 1198 we we PRP 1199 1203 have have VBP 1204 1216 demonstrated demonstrate VBN 1217 1221 that that IN 1222 1226 both both CC 1227 1233 ERK1/2 ERK1/2 NNP 1234 1237 and and CC 1238 1241 p38 p38 NN 1242 1250 cascades cascade NNS 1251 1255 play play VBP 1256 1257 a a DT 1258 1261 key key JJ 1262 1266 role role NN 1267 1269 in in IN 1270 1273 the the DT 1274 1284 production production NN 1285 1287 of of IN 1288 1292 IL-8 il-8 NN 1293 1295 by by IN 1296 1305 monocytes monocyte NNS 1306 1309 and and CC 1310 1313 PMN pmn NN 1314 1324 stimulated stimulate VBN 1325 1329 with with IN 1330 1339 bacterial bacterial JJ 1340 1349 fractions fraction NNS 1349 1350 . . .